| Anifrolumab

Anifrolumab is a monoclonal antibody used for the treatment of systemic lupus erythematosus.It binds to the type I interferon receptor, blocking the activity of type I interferons such as interferon-α and interferon-β.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	50	25	12.5	6.25	3.12	1.56	0.78	0
OD	2.72	1.63	0.9	0.54	0.35	0.27	0.22	0.08
Corrected OD	2.64	1.55	0.82	0.46	0.27	0.19	0.14	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of HumanAnifrolumab spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	92
Plasma(n=8)	92-105	96
Cell Culture Supernates(n=8)	95-110	99

 

| Linearity

Samples were spiked with high concentrations of HumanAnifrolumab and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-98	88-96	90-110
	Average (%)	91	92	97
1 : 4	Range (%)	89-102	87-108	105-115
	Average (%)	91	95	105